Composite

Part:BBa_K4509769

Designed by: MARIE JYOTSNA JAWAHAR, Rhea Harry William, Vibha Murali   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 10:16, 28 September 2022 by Registry (Talk | contribs)

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Cell surface aphA with constitutive promoter

This construct consists of strong promoter and strong RBS combination (BBa_K880005), cell surface tag (BBa_K103006) linked to aphA gene(BBa_K4509369) and GFP reporter attached to terminator (BBa_K259006). OmpA Linker tag helps to express acid phosphatase enzyme(aphA) on the cell surface. The reporter molecule attached will support in tracking the location of the protein and determining gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 966


[edit]
Categories
Parameters
None