Composite
Part:BBa_K4509469
Designed by: Vibha Murali, Rhea Harry William, MARIE JYOTSNA JAWAHAR Group: iGEM22_REC-CHENNAI (2022-09-28)
csgD and OmpR234 Dual Expression
This composite part consists of a strong promoter(BBa_J23118),a strong RBS(BBa_B0034),transcriptional regulators of two curli operons - csgD (BBa_K805015) and OmpR234 (BBa_K342003) and a double terminator (BBa_B0015). The strong promoter and strong RBS are used for the overexpression of the desired proteins. By overexpressing both the proteins, curli fiber production in Escherichia coli K-12 can be increased.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 877
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |