Part:BBa_K4322000

Fibrin Knob Domain (Alpha)

Fibrin is an insoluble protein that is produced in animals in response to bleeding. Fibrin arranges into long fibrous chains that form on the surfaces of activated platelets; together, they produce clots that hinder further blood loss. The polymerization of these long fibrous chains relies on the interaction between N-terminal A- and B-knobs and corresponding a- and b-holes in the gamma- and beta-modules of fibrin [1]. This documented part, when fused with protein csgA of the curli operon and chromoproteins, will allow non-covalent crosslinking to occur between chromoprotein csgA fusion nanofibers [2]. The interaction between the knob and hole domain of fibrin-csgA fusion proteins will also allow for increased stability in microbial-ink hydrogel production [2] This part contains the fibrin alpha knob domain.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]