Composite

Part:BBa_K4399009:Design

Designed by: Duoqing LIN   Group: iGEM22_Worldshaper-Shanghai   (2022-09-27)
Revision as of 03:00, 28 September 2022 by Htclamm (Talk | contribs)

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PLexA35S-SbMYB75-Thsp18.2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 17
    Illegal PstI site found at 1437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 17
    Illegal PstI site found at 1437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1371
    Illegal BamHI site found at 805
    Illegal BamHI site found at 1107
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 17
    Illegal PstI site found at 1437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 17
    Illegal PstI site found at 1437
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Induciable transcription of SbMYB75 (BBa_K4399003) is initiated by PLexA35S (BBa_K3900024) and terminated by Thsp18.2 (BBa_K4399006).


Source

The sequence is based on BBa_K3900024, BBa_K4399003 and BBa_K4399006.

References