Coding
RecA_ESR1

Part:BBa_K4260111

Designed by: Melchor Andrés Soto and Brenda Jiménez   Group: iGEM22_TecCEM   (2022-08-11)
Revision as of 17:29, 27 September 2022 by MelchorSH (Talk | contribs)


RecA mini intein mediated ESR1 protein with OmpA signal peptide, linker and AmilCP.

This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Fig.x:RecA intein behavior when an EDC is present.

POR LLENAR

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Categories
Parameters
None