Coding

Part:BBa_K4165001:Design

Designed by: Amr Ali Gouda   Group: iGEM22_CU_Egypt   (2022-09-27)
Revision as of 17:19, 27 September 2022 by Esraa Elmligy (Talk | contribs) (→‎Design Notes)


Truncated tripartite motif-containing 21 (TRIM21)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 514


Design Notes

The original part on NUDT 2020 parts page has an extra nucleotide at the end so we removed it in order to avoid frameshift. Also, the whole sequence was optimized according to E.Coli BL21

Source

iGEM registry BBa_K3396007

References