Coding

Part:BBa_K4447002:Design

Designed by: Rafael Garcia Gomez   Group: iGEM22_TecMonterrey_GDL   (2022-09-27)
Revision as of 15:59, 27 September 2022 by Rafa1470 (Talk | contribs) (→‎References)


Pcp coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.


Source

The original sequence was reported by Orser et al. (1993). Sequence can be obtained through GenBank (M98557.1).

References

[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli. Journal of bacteriology, 175(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993