Composite

Part:BBa_K4235029

Designed by: Maulik Masaliya   Group: iGEM22_Stony_Brook   (2022-09-21)
Revision as of 14:55, 27 September 2022 by Maulik masaliya (Talk | contribs) (→‎Usage and Biology)

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Ampicillin resistance gene cassette

Usage and Biology

This part is the entire circuit of the Ampicillin Resistance gene BBa_K4235018 which is used within the plasmid and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter BBa_K4235017. This marker was used for the selection of successful transformants after cloning our gene insert BBa_K4235011 in the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 647
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 647
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 647
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 821


[edit]
Categories
//cds
//cds/selectionmarker/antibioticresistance
//chassis/prokaryote
Parameters
None