Coding

Part:BBa_K4256005:Design

Designed by: Yue Lu   Group: iGEM22_UCAS-China   (2022-09-27)
Revision as of 14:32, 27 September 2022 by Registry (Talk | contribs)

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comt (caffeate O-methyltransferase) from A. thaliana (optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BamHI site found at 498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part involves in the production of ferulic acid from L-tyrosine. Considering that the original sequence is from a plant species, we need to codon optimiz it.


Source

Source: Arabidopsis thaliana (thale cress). Reference: Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, etc. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):823-6.

References