Coding
Part:BBa_K4256005:Design
Designed by: Yue Lu Group: iGEM22_UCAS-China (2022-09-27)
comt (caffeate O-methyltransferase) from A. thaliana (optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 563
Illegal BamHI site found at 498 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part involves in the production of ferulic acid from L-tyrosine. Considering that the original sequence is from a plant species, we need to codon optimiz it.
Source
Source: Arabidopsis thaliana (thale cress). Reference: Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, etc. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):823-6.