Device

Part:BBa_K4170016

Designed by: Alexandros Giannopoulos Dimitriou   Group: iGEM22_Thessaloniki_Meta   (2022-09-27)
Revision as of 12:43, 27 September 2022 by Registry (Talk | contribs)

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SUMO-LbuCas13a coding device under T7 promoter

This plasmid contains all the basic genetic parts required for the bacterial expression of codon-optimized LbuCas13a protein in fusion with small ubiquitin-like modifier (SUMO) protein which favors the increased expression and solubility of the recombinant LbuCas13a protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1453
    Illegal BglII site found at 5047
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1421
    Illegal AgeI site found at 1910
    Illegal AgeI site found at 3125
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
Parameters
None