Composite
XDH
Part:BBa_K4324001:Design
Designed by: Chris Yoo Group: iGEM22_TheKingsSchool_AU_HS (2022-09-27)
lacPO-RBS-XDH-T1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 700
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 357
Design Notes
The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.
Source
LacPO:
- RBS:
- CDS: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - XYL2
gene - from Uniprot
- Terminator: