Coding
PK
Part:BBa_K4324103:Design
Designed by: Chris Yoo Group: iGEM22_TheKingsSchool_AU_HS (2022-09-27)
Phosphoketolase from B. lactis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 116
Illegal BamHI site found at 413 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1818
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.
Source
XFP gene from Bifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314)