Coding
XR
Part:BBa_K4324100:Design
Designed by: Chris Yoo Group: iGEM22_TheKingsSchool_AU_HS (2022-09-27)
NAD(P)H-dependent D-xylose reductase from S. stipitis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 747
Design Notes
The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.
Source
XYL1 gene of Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
References
1. https://www.uniprot.org/uniprotkb/P31867/entry 2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.307