Composite
XR
Part:BBa_K4324000:Design
Designed by: Chris Yoo Group: iGEM22_TheKingsSchool_AU_HS (2022-09-21)
lacPO-RBS-XR-T1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 827
Design Notes
The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.
Source
- Promoter-Operator: lacPO - RBS: - CDS: Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) - XYL1 gene - from UniProt - Terminator: