Primer
VR4

Part:BBa_K176048:Design

Designed by: Jiayi Dou, Hao Jiang   Group: iGEM09_USTC   (2009-08-13)
Revision as of 09:24, 13 August 2009 by Horigen (Talk | contribs)

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Reverse primer VR4 (bind to K176038), member of VR3~VR12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Main parameters for picking VR3~VR12:

Size: 20 bp (N19 + G or C)

Tm: VF2 Tm ±1℃

GC%: 50%

Max Self Complementarity: 3 bp

Max 3' Self Complementarity: 2 bp

Max Pair Complementarity: 3 bp

Max 3' Pair Complementarity: 2 bp

Max 3' Stability: 3.36 kcal/mol

Max Template(pSB1A3) Mispriming: 6 bp

Max Mispriming With Other VR3~VR12 Sites: 4 bp

Source

Reverse complement of K176038. Picked from random sequences.

References