Part:BBa_K4235016

pET His6 LIC cloning vector (2Bc-T)

The Human Protein S gene PROS1 () was inserted into this LIC cloning vector and was transformed into E.coli strains Bl21 and origami B for expression.

This vector contains the following parts:

• T7 Promoter: BBa_J64997
  
• RBS: BBa_K2927001
  
• Ampicillin resistance cassette (AmpR promoter+CDS): BBa_K4235029
  
• T7 terminator: BBa_B0016

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 4105
  Illegal PstI site found at 750
  Illegal PstI site found at 4090
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4543
  Illegal PstI site found at 750
  Illegal PstI site found at 4090
  Illegal NotI site found at 4081
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3990
  Illegal BamHI site found at 4207
  Illegal XhoI site found at 4098
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 4105
  Illegal PstI site found at 750
  Illegal PstI site found at 4090
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 4105
  Illegal PstI site found at 750
  Illegal PstI site found at 4090
  Illegal NgoMIV site found at 3076
  Illegal NgoMIV site found at 3430
  Illegal NgoMIV site found at 3590
  Illegal NgoMIV site found at 3958
  Illegal NgoMIV site found at 4054
  Illegal NgoMIV site found at 4237
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 924
  Illegal BsaI.rc site found at 4029
  Illegal SapI.rc site found at 2006