Composite

Part:BBa_K4260002

Designed by: Brenda Jimenez, Karen Marín, Melchor Soto   Group: iGEM22_TecCEM   (2022-09-26)
Revision as of 00:20, 27 September 2022 by Registry (Talk | contribs)

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ESR1: Estrogen Receptor 1 coding cassette (Composite part)

The TecCEM team 2022 designed the biobrick construct cassette for the IPTG inducible expression of the Human Estrogen Receptor Alpha (hERa), this is a receptor protein which aim in our biosensor project is to bind to endocrine disrupting chemicals. Therefore, we used the genomic coding sequence of Homo sapiens Estrogen Receptor 1 (ESR1) adding a linker (GGGGSC) to ensure its binding to chitosan and a histidine tag for a nickel column purification. Its expression was set under control of the T7 promoter and lac operator being inducible by IPTG. Finally, we added a periplasmic signal peptide Omp A. The whole sequence expresses the receptor protein with a linker, setting an specific rotation so that the ligand binding domain (LBD) to be in a reachable position. This ensures the correct positioning of the molecule and therefore, an efficient capture of the EDCs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1997
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None