Coding

Part:BBa_K196004:Design

Designed by: Laetitia Warny   Group: iGEM09_ULB-Brussels   (2009-08-13)
Revision as of 08:08, 13 August 2009 by Registry (Talk | contribs)

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HfsG + HfsH proteins from Caulobacter crescentus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal AgeI site found at 379
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt [3]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.


Source

This sequence comes from Caulobacter crescentus.

References