DNA
Making Bio

Part:BBa_K4329001

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-24)
Revision as of 20:09, 26 September 2022 by Dawang (Talk | contribs) (→‎What is it?)


Overexpression of dethiobiotin synthase and biotin synthase in E. coli

what is it?

In order to alter the metabolic pathway of E. coli and tend to a more productive way of Biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of dethiobiotin synthase and biotin synthase. This plasmid is regulated by T7 promoter, Lac operator, and T7 terminator. With IPTG, RNA ligase will converge with T7 promoter which will then lead to the production of mRNA. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating large amount of biotin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
//cds/biosynthesis
//cds/enzyme
Parameters
function