Coding

Part:BBa_K4432021:Design

Designed by: Paul Weimer   Group: iGEM22_Evry_Paris-Saclay   (2022-09-26)
Revision as of 18:51, 26 September 2022 by PaulW (Talk | contribs)

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CrtO: β−carotene ketolase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically optimised for Escherichia coli with the help of IDT’s online Codon Optimization Tool based on the original CrtO of Dietzia psychralcaliphila ILA-1 sequence (GenBank: AWH95158.1).


Source

DNA synthesis

References