Coding

Part:BBa_K4259000:Design

Designed by: Jennifer Adami   Group: iGEM22_Leiden   (2022-09-26)
Revision as of 16:19, 26 September 2022 by Registry (Talk | contribs)

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Cup1 for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

two overhang sequences with the restriction enzymes BamHI and NdeI were designed and added to the sequence


Source

aattagttaactacgcatctggctgctcttgtggtgctgactgcaaatgtgcttccgaaactgaatgcaaat

References