Composite

Part:BBa_K200010

Designed by: David Roche, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 15:24, 12 August 2009 by Registry (Talk | contribs)

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TaqI Composite

This sequence codes for the restriction enzyme TaqI. This enzyme is Dam methylation sensitive.

The recognition sequence is TCGA. TaqI is a 4 base cutting enzyme, leaving a 2 base overhang.

The cutting site is as shown:

TCGA -> T CGA

AGCT -> AGC T

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 36
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


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Categories
Parameters
None