Coding

Part:BBa_K4220011

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-25)
Revision as of 13:33, 26 September 2022 by Flora778 (Talk | contribs)

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alpha factor sp-V5-RTAC

Introduction:

RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.

Composition:

1.Functional structure: ZNRF3+linker+LGR4+human IgGH3 Fc

2.alpha factor sp: the signaling peptide of alpha factor in the yeast mating pathway


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 471
    Illegal XbaI site found at 1404
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1407
    Illegal BamHI site found at 2288
    Illegal XhoI site found at 2279
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 471
    Illegal XbaI site found at 1404
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 471
    Illegal XbaI site found at 1404
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
    Illegal AgeI site found at 2391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 283
    Illegal BsaI site found at 1604
    Illegal BsaI.rc site found at 1292


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Parameters
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