Coding

Part:BBa_K4220007

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-25)
Revision as of 13:28, 26 September 2022 by Flora778 (Talk | contribs) (→‎Results)


P2Y2-P2A-Galpha

Introduction

P2Y2 is the receptor of eATP which is chosen as our signal molecule to target tumor microenvironment (TME).

By transferring this sequence into the yeast, P2Y2 (human GPCR) and Galpha protein can be produced at the same time with the leakage of P2A peptide.

In order to improve the capability for P2Y2 to be responsiveness to extracellular ATP (eATP), substitutions (K240N and F307S) will be applied.

Results

The production of P2Y2 and Galpha:

P2Y2:

caption

Galpha:

caption

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1095
    Illegal AgeI site found at 389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 570
    Illegal SapI site found at 615


[edit]
Categories
Parameters
None