Coding

Part:BBa_K4220000

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-25)
Revision as of 12:52, 26 September 2022 by Flora778 (Talk | contribs) (→‎The design and improvement of RTAC:)


FZD5 sp-V5-RTAC


Introduction:

RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.

Composition:

ZNRF3+linker+LGR4+human IgGH3 Fc

The design and improvement of RTAC:

1.Better interaction with 4 RSPOs--changing the length of linker: caption

2.Better secretion capability--changing the type of signaling peptide

3.Better stability--adding an IGg Fc tag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1254
    Illegal BamHI site found at 2171
    Illegal XhoI site found at 2162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI site found at 1451
    Illegal BsaI.rc site found at 1139


[edit]
Categories
Parameters
None