Part:BBa_K4291016
CueR-CopA-amilGFP
J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro-amilGFP- rrnB T1 ter- T7Te ter
Characterization by Canton_HS
BBa_K4291016
Name: CuR-CopA-amilGFP Base Pairs: 1703 bp Origin: E.coli, genome Properties: Monitor the Cu concentration
Usage and biology
The plasmid was designed to monitor the cooper concentration using the amilGFP intensity. The CueR which belongs to mercury resistance regulator (MerR) family response the mental ions including cooper and silver. Meanwhile, the CueR control the expression of CopA promoter. In the MerR family, there are other metal-responsive metal-regulators including ZntR, CadR, CoaR which response to the heavy metal zinc, cadmium and cobalt, respectively. All of the metal-regulators has the same property that these regulators could balance the heavy metal ions in bacteria via regulating the transcription of heavy-metal-resistance genes.
The components of this plasmid are described as follows:
BBa_K4291012
Name: CueR Base Pairs: 408 bp Origin: E.coli, genome Properties: regulate the cooper efflux
Usage and biology
BBa_K4291012 is an encoding sequence of cooper efflux regulator (CuR) which is sensitive to metal ions such as Cu+, Ag+, Au+. In addition, CuR controls the transcription of copA in response the cooper salt.
BBa_K4291013
Name: CopA promoter Base Pairs: 287 bp Origin: E.coli, genome Properties: Cu+-transporting ATPase pump
Usage and biology
BBa_K4291013 is an encoding sequence copA which is a cooper-transporter P-type ATPase pump. The expression of copA is improved when exposed to metal ions such as silver and cupric ions due to eliminating the CuR effect.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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