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Part:BBa_K4247013
Minispidroin_NT-4rep-CT-SnoopTag_N-6His
This part codes for the full minispidroin protein, a highly soluble spider silk protein, with a 6x His-tag in the N-terminus. This is a composite part consisting of the following basic parts: BBa_K4247005 (Minispidroin_NT_N-6His), 2 repeats of BBa_K4247001 (Minispidroin_2rep), BBa_K4247002 (Minispidroin_CT) and and BBa_K4247008 (SnoopTag). BBa_K4247012 contains the coding sequence for the full minispidroin protein with 4 repeats of the central repetitive domain and a 6His-tag in the N-terminus of the protein.
This part is one of a collection of compatible minispidroin parts: BBa_K4247000 (Minispidroin_NT), BBa_K4247001 (Minispidroin_2rep), BBa_K4247002 (Minispidroin_CT), BBa_K4247004 (Minispidroin_NT-2rep-CT), BBa_K247005 (Minispidroin_NT_N-6His), BBa_K247007 (Minispidroin_NT-2rep-CT_N-6His), BBa_K247010 (Minispidroin_NT-2rep-CT-SnoopTag_N-6His), BBa_K247011 (Minispidroin_NT-4rep-CT), BBa_K247012 (Minispidroin_NT-4rep-CT_N-6His), BBa_K247013 (Minispidroin_NT-4rep-CT-SnoopTag_N-6His).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 493
Illegal PstI site found at 499
Illegal PstI site found at 514
Illegal PstI site found at 568
Illegal PstI site found at 586
Illegal PstI site found at 745
Illegal PstI site found at 751 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 493
Illegal PstI site found at 499
Illegal PstI site found at 514
Illegal PstI site found at 568
Illegal PstI site found at 586
Illegal PstI site found at 745
Illegal PstI site found at 751 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 493
Illegal PstI site found at 499
Illegal PstI site found at 514
Illegal PstI site found at 568
Illegal PstI site found at 586
Illegal PstI site found at 745
Illegal PstI site found at 751 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 493
Illegal PstI site found at 499
Illegal PstI site found at 514
Illegal PstI site found at 568
Illegal PstI site found at 586
Illegal PstI site found at 745
Illegal PstI site found at 751 - 1000COMPATIBLE WITH RFC[1000]
None |