Coding

Part:BBa_K4247013

Designed by: Matteo Soana   Group: iGEM22_UCopenhagen   (2022-09-26)
Revision as of 10:29, 26 September 2022 by Registry (Talk | contribs)

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Minispidroin_NT-4rep-CT-SnoopTag_N-6His

This part codes for the full minispidroin protein, a highly soluble spider silk protein, with a 6x His-tag in the N-terminus. This is a composite part consisting of the following basic parts: BBa_K4247005 (Minispidroin_NT_N-6His), 2 repeats of BBa_K4247001 (Minispidroin_2rep), BBa_K4247002 (Minispidroin_CT) and and BBa_K4247008 (SnoopTag). BBa_K4247012 contains the coding sequence for the full minispidroin protein with 4 repeats of the central repetitive domain and a 6His-tag in the N-terminus of the protein.

This part is one of a collection of compatible minispidroin parts: BBa_K4247000 (Minispidroin_NT), BBa_K4247001 (Minispidroin_2rep), BBa_K4247002 (Minispidroin_CT), BBa_K4247004 (Minispidroin_NT-2rep-CT), BBa_K247005 (Minispidroin_NT_N-6His), BBa_K247007 (Minispidroin_NT-2rep-CT_N-6His), BBa_K247010 (Minispidroin_NT-2rep-CT-SnoopTag_N-6His), BBa_K247011 (Minispidroin_NT-4rep-CT), BBa_K247012 (Minispidroin_NT-4rep-CT_N-6His), BBa_K247013 (Minispidroin_NT-4rep-CT-SnoopTag_N-6His).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 493
    Illegal PstI site found at 499
    Illegal PstI site found at 514
    Illegal PstI site found at 568
    Illegal PstI site found at 586
    Illegal PstI site found at 745
    Illegal PstI site found at 751
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 493
    Illegal PstI site found at 499
    Illegal PstI site found at 514
    Illegal PstI site found at 568
    Illegal PstI site found at 586
    Illegal PstI site found at 745
    Illegal PstI site found at 751
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 493
    Illegal PstI site found at 499
    Illegal PstI site found at 514
    Illegal PstI site found at 568
    Illegal PstI site found at 586
    Illegal PstI site found at 745
    Illegal PstI site found at 751
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 493
    Illegal PstI site found at 499
    Illegal PstI site found at 514
    Illegal PstI site found at 568
    Illegal PstI site found at 586
    Illegal PstI site found at 745
    Illegal PstI site found at 751
  • 1000
    COMPATIBLE WITH RFC[1000]


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