Part:BBa_K4202004

TSLV-BS-CA

Usage and Biology

Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, α-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO₂ to rapidly produce bicarbonate (HCO₃^-) and protons (H⁺).

Chemical reaction equation: H₂O+CO₂↔HCO₃^-+H⁺

Bicarbonate (HCO₃^-) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca²⁺) in the extracellular medium, the bicarbonate can combine to the Ca²⁺ to form calcium carbonate precipitates. In our project, the calcium carbonate precipitate can accumulate in the tiny cracks of the stone artifacts, filling the cracks and providing support.

This part derivers from BBa_K2232000 We replace codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are more compatible with the codon usage bias of Bacillus subtilis, avoiding the emergence of rare codons

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 540
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 166
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 535