Generator

Part:BBa_K4202007:Experience

Designed by: LIAOLIAO GAO   Group: iGEM22_ZJU-China   (2022-09-25)
Revision as of 03:01, 26 September 2022 by SinubilovesCola (Talk | contribs) (→‎Applications of BBa_K4202007)


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Applications of BBa_K4202007

we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor.

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Fig 1-1Characterization of Oxygen-limitation induced promotor by SDS-PAGE

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