Other
ccdA

Part:BBa_K196000:Design

Designed by: Laetitia Warny   Group: iGEM09_ULB-Brussels   (2009-08-11)
Revision as of 08:03, 11 August 2009 by Laetitia (Talk | contribs) (→‎Source)

CcdA antidote with the mob promoter (reverse)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Initially, the ccdA sequence was introduced in the plasmid in the reversed way. In order to leave the possibility to use it in both ways, we also designed this part in the forward way.


Source

This part comes from the StabyTM plasmid, designed by Delphi Genetics [http://www.delphigenetics.com].

References

CEDRIC Y. SZPIRER AND MICHEL C. MILINKOVITCH, Separate-component-stabilization system for protein and DNA production without the use of antibiotics, BioTechniques 38:775-781 (May 2005) SZPIRER ET AL., Mobilization Function of the pBHR1 Plasmid, a Derivative of the Broad-Host-Range Plasmid pBBR1, Journal of bacteriology, Mar. 2001, p. 2101–2110 Vol. 183, No. 6