Coding

Part:BBa_K4348057:Design

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-09-25)
Revision as of 17:20, 25 September 2022 by Jonathancheng26 (Talk | contribs) (→‎Design Notes)


SUMO-his


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 281
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 281
    Illegal BglII site found at 170
    Illegal BamHI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains an EcoRI site, so standard biobrick assembly is not possible

Source

Saccharomyces cerevisiae

References