Coding

Part:BBa_K4348057

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-09-25)
Revision as of 17:18, 25 September 2022 by Registry (Talk | contribs)

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SUMO-his

The SUMO protein is a small, very soluble protein that when fused with your protein of interest, will make the protein more soluble on average. It can easily be cleaved off later with a protease. This is a useful tool if your protein is aggregating or misfolding into inclusion bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 281
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 281
    Illegal BglII site found at 170
    Illegal BamHI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None