Part:BBa_K4219000:Design
Evolutionary-stabilized optimized GFP reporter protein
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1004 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 29
Illegal NheI site found at 52
Illegal SpeI site found at 1005
Illegal PstI site found at 1019
Illegal NotI site found at 7
Illegal NotI site found at 1012 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1005 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 1005
Illegal PstI site found at 1019 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of this part was determined by utilizing the ESO software, developed by Staubility, TAU representative group for the iGEM 2020 competition. In order to validate that the optimization process has worked properly, an evolutionary experiment, checking whether a significant difference in the evolutionary stability between the original non-optimized part and the newly-optimized one might be witnessed, had been designed. Throughout which, we have measured the fluorescence levels of both parts (encoding for the GFP protein) and checked whether the loss of fluorescence (indicating loss of functionality and a decreased evolutionary stability) of the original part was significantly faster than that of the optimized one.
Source
The source of this part is, as already stated, the BBa_K079050 construct, stored in the 23G position, plate 3 of the iGEM 2021 Kit Distribution.