Part:BBa_K4202004

TSLV-BS-CA

Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, α-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO₂ to rapidly produce bicarbonate (HCO₃^-) and protons (H+). H2O+CO2↔HCO3−+H+ Bicarbonate (HCO3-) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca2+) in the extracellular medium with which the bicarbonate can combine to form calcium carbonate precipitates. The calcium carbonate precipitate can accumulate in the microfractures of the stone artifacts, filling the cracks and providing support.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 540
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 166
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 535