Composite

Part:BBa_K4463002

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)
Revision as of 10:49, 25 September 2022 by Qiaowa (Talk | contribs)


SUMO-CsgA-SmtA

CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and SmtA. Improved parts is based on part <a href="https://parts.igem.org/Part:BBa_K519010" title="BBa_K519010"></a>

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 863
    Illegal AgeI site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
chassisE.coli