Translational_Unit

Part:BBa_K4158009

Designed by: Sota Okuda, Nanami Onishi   Group: iGEM22_Waseda_Tokyo   (2022-09-19)
Revision as of 06:14, 25 September 2022 by S Okuda (Talk | contribs)


RBS-edcR

RBS and coding site for the edcR　protein with TEV site and His tag. edcR binds to edcR regulator and works as repressor. 17β-estradiol binds to edcR and inhibit its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
    Illegal XhoI site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 308
    Illegal AgeI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None