Device

Part:BBa_K4175010:Design

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-09-25)
Revision as of 06:01, 25 September 2022 by JasLee24 (Talk | contribs) (→‎Source)

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IL-6R-Notch-Gal4KRAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
    Illegal BamHI site found at 76
    Illegal BamHI site found at 1194
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
    Illegal NgoMIV site found at 1432
    Illegal NgoMIV site found at 2423
    Illegal NgoMIV site found at 2558
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been codon optimized for human.


Source

human extracellular IL-6R (BBa_K4175002), Notch core (BBa_K4175001), ZF_GAl4_KRAB (BBa_K2446037)

References