Device

Part:BBa_K4175010

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-09-25)
Revision as of 05:39, 25 September 2022 by Registry (Talk | contribs)

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IL-6R-Notch-Gal4KRAB

When interleukin-6 (IL-6) concentration is high to an extent, IL-6 will bind to IL-6 receptor (IL-6R) (BBa_K4175002). Then, the Notch core domain (BBa_K4175001) would undergo intracellular proteolytic cleavage. As a result, the Gal4KRAB domain (BBa_K2446037) will be released into the cytosol and bound to the UAS-pSV40 (BBa_K4040033) in the nucleus. The expression of gene controlled by pSV40 promoter will thus be inhibited.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
    Illegal BamHI site found at 76
    Illegal BamHI site found at 1194
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1294
    Illegal PstI site found at 1519
    Illegal PstI site found at 1560
    Illegal PstI site found at 1606
    Illegal PstI site found at 2638
    Illegal NgoMIV site found at 1432
    Illegal NgoMIV site found at 2423
    Illegal NgoMIV site found at 2558
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologyHuman