Coding
aphA
Part:BBa_K4509369
Designed by: Rhea Harry William, MARIE JYOTSNA JAWAHAR, Vibha Murali Group: iGEM22_REC-CHENNAI (2022-09-24)
Acid phosphatase (aphA)
Acid phosphatase coding sequence without promoter, RBS or terminator from E.coli K12. Some neutral point mutations have been made to this sequence to ensure its compatibility with all assembly standards. This bacterium's aphA gene was chosen to make sure it is effectively expressed in E.coli. When expressed, aphA can be assayed using phosphate substrates like glycerol-2-phosphate, disodium phenyl phosphate, phytic acid etc. The quantity of substrate consumed and free phosphate produced is directly proportional to the quantity of the enzyme present.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 433
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Categories
Parameters
None |