Coding
aphA

Part:BBa_K4509369

Designed by: Rhea Harry William, MARIE JYOTSNA JAWAHAR, Vibha Murali   Group: iGEM22_REC-CHENNAI   (2022-09-24)
Revision as of 16:59, 24 September 2022 by Registry (Talk | contribs)

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Acid phosphatase (aphA)

Acid phosphatase coding sequence without promoter, RBS or terminator from E.coli K12. Some neutral point mutations have been made to this sequence to ensure its compatibility with all assembly standards. This bacterium's aphA gene was chosen to make sure it is effectively expressed in E.coli. When expressed, aphA can be assayed using phosphate substrates like glycerol-2-phosphate, disodium phenyl phosphate, phytic acid etc. The quantity of substrate consumed and free phosphate produced is directly proportional to the quantity of the enzyme present.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 433


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Categories
Parameters
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