DNA
Biotinylat

Part:BBa_K4329000

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-22)
Revision as of 15:55, 24 September 2022 by TinaHe (Talk | contribs)


TurboID-N_AgrD-AIP

What is it?

This part includes four substance: TurboID, AIP, biotin,and N-AgrD. TurboID, a pair of biotin ligases, was designed to overcome the problem of slow existing biotin ligases. Using yeast display directed evolution techniques, the labeling time will be shorted to less than 10 minutes. This enables probing of dynamic biological processes with higher temporal resolution. AIP, autoinducing peptide, is a chemical released by S.aureus as it activates quorum sensing. N-AgrD is also a chemical involved in the cycle of virulence production, and it will direct TurboID toward the target site, AgrB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
Parameters
function
protein