Coding

Part:BBa_K4194007

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)
Revision as of 12:10, 24 September 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


EGFP（+LVA）

a strong reporter. EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Ex:488nm Em :507nm EC :55,900(M-1 cm-1) QY:0.6 Brightness:33.54 pKa:6.0 Maturation:25.0min Lifetime:2.6ns

A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
colorgreen
emission507
excitation488
tagssrA-LVA