Reporter
SRTF1 repo
Part:BBa_K4158010:Design
Designed by: Eri Ito Group: iGEM22_Waseda_Tokyo (2022-09-21)
Psrtf1-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 13
Illegal NheI site found at 36 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 826
Illegal XhoI site found at 529 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
If you want to get the same plasmid that we experimented with, construct the plasmid along the way below.
1. Prepare pACYC184 cloning vector and this part as BioBrick.
2. Restriction and insertion cloning with pACYC184 and this BioBrick productions and restriction enzymes HindIII and BamHI.
Source
test