Plasmid_Backbone
Part:BBa_K4501022:Design
Designed by: Pau Marín Escudero Group: iGEM22_Barcelona_UB (2022-09-22)
pJUMP lentiviral transfer plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 5061
Illegal suffix found at 5105 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5061
Illegal SpeI site found at 5106
Illegal PstI site found at 5120
Illegal NotI site found at 5067
Illegal NotI site found at 5113 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5061
Illegal BglII site found at 33
Illegal BglII site found at 74
Illegal BglII site found at 3682 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5061
Illegal suffix found at 5106 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5061
Plasmid lacks a suffix.
Illegal XbaI site found at 5076
Illegal SpeI site found at 5106
Illegal PstI site found at 5120
Illegal NgoMIV site found at 1895 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2693
Illegal SapI.rc site found at 4194
Design Notes
We had to remove the BsmBI recognition sites of the MSCV modules.
Source
Designed by ourselves.