DNA
Part:BBa_K4115011:Design
Designed by: Renjie Yu Group: iGEM22_ShanghaiTech_China (2022-09-21)
NSI-DS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 442
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 442
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 442
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 442
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
NSI-DS is placed downstream of the aimed fragments when constructing our plasmids needed for HL7942 and UTEX2973.
Source
The sequence is gained from the genome of Synechococcus 2973 and the fragment we use is synthesized by the company.