Coding

Part:BBa_K4361301

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-19)
Revision as of 10:36, 22 September 2022 by Larsvdb (Talk | contribs)


BlcR D37V

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R1 (Part:BBa_K4361200) and F1.2 D37V (Part:BBa_K4361202). For this mutant, the aspartic acid in position 37 has been changed to valine by mutating the GAC codon to GTG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • A 50 > C, silent mutation

Sequencing data only contained this part's sequence up until and including nucleotide 421. All nucleotides after are presumed to be identical to those found in the original codon optimized BlcR, Part:BBa_K4361100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589


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