DNA

Part:BBa_K4115007:Design

Designed by: Shenxi Cao   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 14:44, 21 September 2022 by Registry (Talk | contribs)

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NifA_upstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 234
    Illegal NgoMIV site found at 339
    Illegal NgoMIV site found at 556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 331


Design Notes

It is used as a one-sided homology arm.


Source

This sequence is from the Azorhizobium caulinodans ORS571 genome.

References