Composite

Part:BBa_K4430008

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)
Revision as of 02:05, 21 September 2022 by Registry (Talk | contribs)

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GFP-TSP

It is the key part that is responsible for expressing green fluorescent protein (GFP) fusion protein of tailspike protein (TSP) (GFP-TSP). TSP is from the genome of Salmonella phage P22. The tailspikes of P22 and its podoviral relatives display modular structures. The smaller N-terminal particle-binding domain (PBD) of the homotrimeric TSP binds the elongated molecule to the phage head. This segment of around 110 amino-acid residues is highly conserved in sequence between different phages of the same morphology. The much larger C-terminal part of TSP mediates binding to the receptor (receptor-binding domain, RBD). GFP emits green fluorescence under the confocal laser scanning microscope. Under such microscope, green fluorescence on the cell surface of SE after incubation with GFP-TSP proteins indicates specific binding of the proteins to the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


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