Coding

Part:BBa_K4461000:Design

Designed by: YUAN, HSIAO   Group: iGEM22_NYCU-Taipei   (2022-09-20)
Revision as of 16:21, 20 September 2022 by YU-TANG-CHANG (Talk | contribs) (→‎Design Notes)


dusB-fis promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned a larger fragment that included the promoter, then used another pair of primers to clone the promoter specifically. Therefore, we can avoid the length of the unintended bands being too similar to distinguish easily. The sequence we cloned has to include all transcriptional factors for the promoter. Also, we used BBa_B0034 as the RBS, so we didn't clone the RBS from the genomic DNA.

Source

The dusB-fis promoter is from E.coli-K12 MG1655 genomic DNA.

References