![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4115002:Design
Designed by: Shenxi Cao Group: iGEM22_ShanghaiTech_China (2022-09-20)
NifA(nif-specific transcriptional activator)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 136
Illegal PstI site found at 1000 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 136
Illegal PstI site found at 1000 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 637
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 136
Illegal PstI site found at 1000 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 136
Illegal PstI site found at 1000
Illegal NgoMIV site found at 1257
Illegal NgoMIV site found at 1452
Illegal NgoMIV site found at 1527
Illegal AgeI site found at 886 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1095
Illegal SapI site found at 318
Illegal SapI site found at 939
Design Notes
It can be exploited to regulate the expression of nitrogen fixation genes in Azorhizobium caulinodans.
Source
This gene sequence is part of the genome of Azorhizobium caulinodans ORS571. We synthesized this sequence through a commercial synthesis company.