Coding

Part:BBa_K4461000:Design

Designed by: YUAN, HSIAO   Group: iGEM22_NYCU-Taipei   (2022-09-20)
Revision as of 05:49, 20 September 2022 by Registry (Talk | contribs)

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dusB-fis promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned a larger fragment which include the promoter, then use another pair of primer to specify clone the promoter. Therefore we can avoid the length of the unintended bands are too similar to distinct easily. The sequence we cloned have to include all transcriptional factors for the promoter. We used BBa_B0034 as the RBS of the promoter, so we didn't cloned the RBS from the genomic DNA.



Source

The dusB-fis promoter is from E.coli-K12 MG1655 genomic DNA.

References